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vitro cell senescence model normal rat small intestinal epithelial cells  (ATCC)


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    ATCC vitro cell senescence model normal rat small intestinal epithelial cells
    FIGURE 2 H2O2-induced cell <t>senescence</t> in vitro model induced CB1 reduction. (A) Morphological changes showed <t>IEC-6</t> cell senescence by H2O2 treatment (150 mM) in a time-dependent manner (n=3). Scale bar, 100 mm. (B) b-gal staining indicated that positive cells (blue) increased with H2O2 treatment (150 mM) in a time-dependent manner (n=5). Scale bar, 100 mm. (C) qPCR was conducted to confirm the RNA expression of CB1 in H2O2-treated IEC-6 cells. Statistical differences were determined using t-test; a single asterisk (*) indicates p<0.05 compared to the control group. Error bars, SD (n=3). GAPDH was used for the loading control. (D, E) Western blot analysis was conducted to investigate the CB1 expression alteration by H2O2 treatment. The bar graphs represent the optical density quantification of the protein bands for each group (D, E, bottom). Statistical differences were determined using t-test; a single asterisk (*) indicates p<0.05, triple asterisks (***) indicate p<0.001 compared to control group. Error bars, SD (n=3). b-actin was used for the loading control. (F, G) CB1 mRNA expression was detected by qPCR in H2O2-treated IEC-6 cells and in cells treated with the selective CB1 inverse agonist, SR141716A (F), and/or the selective CB1 agonist ACEA (G). Statistical differences were determined using t-test; **p<0.01 and ***p<0.001 compared to control group. #p<0.05 compared to H2O2-treated group. Error bars, SD (n=3).
    Vitro Cell Senescence Model Normal Rat Small Intestinal Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1146 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vitro+cell+senescence+model+normal+rat+small+intestinal+epithelial+cells/IEC-6/pm37693348-58-4-33
    Average 96 stars, based on 1146 article reviews
    vitro cell senescence model normal rat small intestinal epithelial cells - by Bioz Stars, 2026-10
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    1) Product Images from "Cannabinoid receptor type 1 in the aging gut regulates the mucosal permeability via miR-191-5p."

    Article Title: Cannabinoid receptor type 1 in the aging gut regulates the mucosal permeability via miR-191-5p.

    Journal: Frontiers in endocrinology

    doi: 10.3389/fendo.2023.1241097

    FIGURE 2 H2O2-induced cell senescence in vitro model induced CB1 reduction. (A) Morphological changes showed IEC-6 cell senescence by H2O2 treatment (150 mM) in a time-dependent manner (n=3). Scale bar, 100 mm. (B) b-gal staining indicated that positive cells (blue) increased with H2O2 treatment (150 mM) in a time-dependent manner (n=5). Scale bar, 100 mm. (C) qPCR was conducted to confirm the RNA expression of CB1 in H2O2-treated IEC-6 cells. Statistical differences were determined using t-test; a single asterisk (*) indicates p<0.05 compared to the control group. Error bars, SD (n=3). GAPDH was used for the loading control. (D, E) Western blot analysis was conducted to investigate the CB1 expression alteration by H2O2 treatment. The bar graphs represent the optical density quantification of the protein bands for each group (D, E, bottom). Statistical differences were determined using t-test; a single asterisk (*) indicates p<0.05, triple asterisks (***) indicate p<0.001 compared to control group. Error bars, SD (n=3). b-actin was used for the loading control. (F, G) CB1 mRNA expression was detected by qPCR in H2O2-treated IEC-6 cells and in cells treated with the selective CB1 inverse agonist, SR141716A (F), and/or the selective CB1 agonist ACEA (G). Statistical differences were determined using t-test; **p<0.01 and ***p<0.001 compared to control group. #p<0.05 compared to H2O2-treated group. Error bars, SD (n=3).
    Figure Legend Snippet: FIGURE 2 H2O2-induced cell senescence in vitro model induced CB1 reduction. (A) Morphological changes showed IEC-6 cell senescence by H2O2 treatment (150 mM) in a time-dependent manner (n=3). Scale bar, 100 mm. (B) b-gal staining indicated that positive cells (blue) increased with H2O2 treatment (150 mM) in a time-dependent manner (n=5). Scale bar, 100 mm. (C) qPCR was conducted to confirm the RNA expression of CB1 in H2O2-treated IEC-6 cells. Statistical differences were determined using t-test; a single asterisk (*) indicates p<0.05 compared to the control group. Error bars, SD (n=3). GAPDH was used for the loading control. (D, E) Western blot analysis was conducted to investigate the CB1 expression alteration by H2O2 treatment. The bar graphs represent the optical density quantification of the protein bands for each group (D, E, bottom). Statistical differences were determined using t-test; a single asterisk (*) indicates p<0.05, triple asterisks (***) indicate p<0.001 compared to control group. Error bars, SD (n=3). b-actin was used for the loading control. (F, G) CB1 mRNA expression was detected by qPCR in H2O2-treated IEC-6 cells and in cells treated with the selective CB1 inverse agonist, SR141716A (F), and/or the selective CB1 agonist ACEA (G). Statistical differences were determined using t-test; **p<0.01 and ***p<0.001 compared to control group. #p<0.05 compared to H2O2-treated group. Error bars, SD (n=3).

    Techniques Used: In Vitro, Staining, RNA Expression, Control, Western Blot, Expressing

    FIGURE 3 Intestinal permeability and tight junction protein expression were regulated by CB1 signaling modulation. (A) Morphological changes showed IEC-6 cell senescence by H2O2 and SR141716A treatment (n=5). Scale bar, 100 mm. (B) The TEER values were detected in H2O2-treated IEC-6 cells and cells treated with the CB1 inverse agonist SR141716A. The bar graph indicated the TEER values at 24 h (B, right). Statistical differences were determined using t-test; ***p<0.001 compared to control group. Error bars, SD (n=3). (C) Morphological changes showed IEC-6 cell senescence caused by H2O2 and the restoration of cell morphology with ACEA treatment after 24 h and 48 h (n=5). Scale bar, 100 mm. (D) The decreased TEER values caused by H2O2 treatment were increased by co-treatment with the CB1 agonist ACEA. The bar graph presents the TEER values at 24 h after treatment with H2O2 and ACEA (D, right). Statistical differences were determined using t-test; ***p<0.001 compared to control group and ###p<0.001 compared to H2O2- treated group. Error bars, SD (n=3). (E, F) The mRNA expression levels of the tight junction proteins ZO-1, ZO-2, Claudin-2, Claudin-4 and Occludin were detected by RT-PCR in H2O2-treated IEC-6 cells. RT-PCR results showed a decrease in the levels of these tight junction proteins (E) and the bar graph presents the band intensity of the RT-PCR results (F). Statistical differences were determined using t-test; *p<0.05, **p<0.01, and ***p<0.001 compared to control group. Error bars, SD (n = 3). GAPDH was used for the loading control. (G) Decreased RNA expression of ZO-1 by H2O2 was restored by ACEA co-treatment. The bar graphs present the band intensity of PCR results. Statistical differences were determined using t-test; *p<0.05 compared to control group and #p<0.05 compared to H2O2-treated group. Error bars, SD (n = 3).
    Figure Legend Snippet: FIGURE 3 Intestinal permeability and tight junction protein expression were regulated by CB1 signaling modulation. (A) Morphological changes showed IEC-6 cell senescence by H2O2 and SR141716A treatment (n=5). Scale bar, 100 mm. (B) The TEER values were detected in H2O2-treated IEC-6 cells and cells treated with the CB1 inverse agonist SR141716A. The bar graph indicated the TEER values at 24 h (B, right). Statistical differences were determined using t-test; ***p<0.001 compared to control group. Error bars, SD (n=3). (C) Morphological changes showed IEC-6 cell senescence caused by H2O2 and the restoration of cell morphology with ACEA treatment after 24 h and 48 h (n=5). Scale bar, 100 mm. (D) The decreased TEER values caused by H2O2 treatment were increased by co-treatment with the CB1 agonist ACEA. The bar graph presents the TEER values at 24 h after treatment with H2O2 and ACEA (D, right). Statistical differences were determined using t-test; ***p<0.001 compared to control group and ###p<0.001 compared to H2O2- treated group. Error bars, SD (n=3). (E, F) The mRNA expression levels of the tight junction proteins ZO-1, ZO-2, Claudin-2, Claudin-4 and Occludin were detected by RT-PCR in H2O2-treated IEC-6 cells. RT-PCR results showed a decrease in the levels of these tight junction proteins (E) and the bar graph presents the band intensity of the RT-PCR results (F). Statistical differences were determined using t-test; *p<0.05, **p<0.01, and ***p<0.001 compared to control group. Error bars, SD (n = 3). GAPDH was used for the loading control. (G) Decreased RNA expression of ZO-1 by H2O2 was restored by ACEA co-treatment. The bar graphs present the band intensity of PCR results. Statistical differences were determined using t-test; *p<0.05 compared to control group and #p<0.05 compared to H2O2-treated group. Error bars, SD (n = 3).

    Techniques Used: Permeability, Expressing, Control, Reverse Transcription Polymerase Chain Reaction, RNA Expression

    FIGURE 7 Reduction of CB1 in aged intestinal epithelial cells caused tight junction dysfunction. Decreased CB1 is associated with the induction of miR-191-5p. miR-191-5p reduced ZO-1 expression by targeting directly and via activation of p-p65 NF-kB transcription factor.
    Figure Legend Snippet: FIGURE 7 Reduction of CB1 in aged intestinal epithelial cells caused tight junction dysfunction. Decreased CB1 is associated with the induction of miR-191-5p. miR-191-5p reduced ZO-1 expression by targeting directly and via activation of p-p65 NF-kB transcription factor.

    Techniques Used: Expressing, Activation Assay

    Related Articles

    Cell Culture:

    Article Title: Cannabinoid receptor type 1 in the aging gut regulates the mucosal permeability via miR-191-5p.
    Article Snippet: .. Cell culture & in vitro cell senescence model Normal rat small intestinal epithelial cells (IEC-6; cat. no. CRL1592) and the human colonic epithelial adenocarcinoma cell line Caco-2 (cat. no. HTB-37TM) were purchased from American Type Culture Collection (Manassas, VA, USA). .. Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with high glucose (4.5 g/L), 10% heat-inactivated fetal bovine serum, and 100 units/ml penicillin, and 100 mg/ml streptomycin solution (all from Hyclone Laboratories, Logan, UT, USA) at 37 °C in a 5% CO2 air environment.

    In Vitro:

    Article Title: Cannabinoid receptor type 1 in the aging gut regulates the mucosal permeability via miR-191-5p.
    Article Snippet: .. Cell culture & in vitro cell senescence model Normal rat small intestinal epithelial cells (IEC-6; cat. no. CRL1592) and the human colonic epithelial adenocarcinoma cell line Caco-2 (cat. no. HTB-37TM) were purchased from American Type Culture Collection (Manassas, VA, USA). .. Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with high glucose (4.5 g/L), 10% heat-inactivated fetal bovine serum, and 100 units/ml penicillin, and 100 mg/ml streptomycin solution (all from Hyclone Laboratories, Logan, UT, USA) at 37 °C in a 5% CO2 air environment.



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    vitro cell senescence model normal rat small intestinal epithelial cells  (ATCC)
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    ATCC vitro cell senescence model normal rat small intestinal epithelial cells
    FIGURE 2 H2O2-induced cell <t>senescence</t> in vitro model induced CB1 reduction. (A) Morphological changes showed <t>IEC-6</t> cell senescence by H2O2 treatment (150 mM) in a time-dependent manner (n=3). Scale bar, 100 mm. (B) b-gal staining indicated that positive cells (blue) increased with H2O2 treatment (150 mM) in a time-dependent manner (n=5). Scale bar, 100 mm. (C) qPCR was conducted to confirm the RNA expression of CB1 in H2O2-treated IEC-6 cells. Statistical differences were determined using t-test; a single asterisk (*) indicates p<0.05 compared to the control group. Error bars, SD (n=3). GAPDH was used for the loading control. (D, E) Western blot analysis was conducted to investigate the CB1 expression alteration by H2O2 treatment. The bar graphs represent the optical density quantification of the protein bands for each group (D, E, bottom). Statistical differences were determined using t-test; a single asterisk (*) indicates p<0.05, triple asterisks (***) indicate p<0.001 compared to control group. Error bars, SD (n=3). b-actin was used for the loading control. (F, G) CB1 mRNA expression was detected by qPCR in H2O2-treated IEC-6 cells and in cells treated with the selective CB1 inverse agonist, SR141716A (F), and/or the selective CB1 agonist ACEA (G). Statistical differences were determined using t-test; **p<0.01 and ***p<0.001 compared to control group. #p<0.05 compared to H2O2-treated group. Error bars, SD (n=3).
    Vitro Cell Senescence Model Normal Rat Small Intestinal Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vitro+cell+senescence+model+normal+rat+small+intestinal+epithelial+cells/IEC-6/pm37693348-58-4-33
    Average 96 stars, based on 1 article reviews
    vitro cell senescence model normal rat small intestinal epithelial cells - by Bioz Stars, 2026-10
    96/100 stars
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    FIGURE 2 H2O2-induced cell senescence in vitro model induced CB1 reduction. (A) Morphological changes showed IEC-6 cell senescence by H2O2 treatment (150 mM) in a time-dependent manner (n=3). Scale bar, 100 mm. (B) b-gal staining indicated that positive cells (blue) increased with H2O2 treatment (150 mM) in a time-dependent manner (n=5). Scale bar, 100 mm. (C) qPCR was conducted to confirm the RNA expression of CB1 in H2O2-treated IEC-6 cells. Statistical differences were determined using t-test; a single asterisk (*) indicates p<0.05 compared to the control group. Error bars, SD (n=3). GAPDH was used for the loading control. (D, E) Western blot analysis was conducted to investigate the CB1 expression alteration by H2O2 treatment. The bar graphs represent the optical density quantification of the protein bands for each group (D, E, bottom). Statistical differences were determined using t-test; a single asterisk (*) indicates p<0.05, triple asterisks (***) indicate p<0.001 compared to control group. Error bars, SD (n=3). b-actin was used for the loading control. (F, G) CB1 mRNA expression was detected by qPCR in H2O2-treated IEC-6 cells and in cells treated with the selective CB1 inverse agonist, SR141716A (F), and/or the selective CB1 agonist ACEA (G). Statistical differences were determined using t-test; **p<0.01 and ***p<0.001 compared to control group. #p<0.05 compared to H2O2-treated group. Error bars, SD (n=3).

    Journal: Frontiers in endocrinology

    Article Title: Cannabinoid receptor type 1 in the aging gut regulates the mucosal permeability via miR-191-5p.

    doi: 10.3389/fendo.2023.1241097

    Figure Lengend Snippet: FIGURE 2 H2O2-induced cell senescence in vitro model induced CB1 reduction. (A) Morphological changes showed IEC-6 cell senescence by H2O2 treatment (150 mM) in a time-dependent manner (n=3). Scale bar, 100 mm. (B) b-gal staining indicated that positive cells (blue) increased with H2O2 treatment (150 mM) in a time-dependent manner (n=5). Scale bar, 100 mm. (C) qPCR was conducted to confirm the RNA expression of CB1 in H2O2-treated IEC-6 cells. Statistical differences were determined using t-test; a single asterisk (*) indicates p<0.05 compared to the control group. Error bars, SD (n=3). GAPDH was used for the loading control. (D, E) Western blot analysis was conducted to investigate the CB1 expression alteration by H2O2 treatment. The bar graphs represent the optical density quantification of the protein bands for each group (D, E, bottom). Statistical differences were determined using t-test; a single asterisk (*) indicates p<0.05, triple asterisks (***) indicate p<0.001 compared to control group. Error bars, SD (n=3). b-actin was used for the loading control. (F, G) CB1 mRNA expression was detected by qPCR in H2O2-treated IEC-6 cells and in cells treated with the selective CB1 inverse agonist, SR141716A (F), and/or the selective CB1 agonist ACEA (G). Statistical differences were determined using t-test; **p<0.01 and ***p<0.001 compared to control group. #p<0.05 compared to H2O2-treated group. Error bars, SD (n=3).

    Article Snippet: Cell culture & in vitro cell senescence model Normal rat small intestinal epithelial cells (IEC-6; cat. no. CRL1592) and the human colonic epithelial adenocarcinoma cell line Caco-2 (cat. no. HTB-37TM) were purchased from American Type Culture Collection (Manassas, VA, USA).

    Techniques: In Vitro, Staining, RNA Expression, Control, Western Blot, Expressing

    FIGURE 3 Intestinal permeability and tight junction protein expression were regulated by CB1 signaling modulation. (A) Morphological changes showed IEC-6 cell senescence by H2O2 and SR141716A treatment (n=5). Scale bar, 100 mm. (B) The TEER values were detected in H2O2-treated IEC-6 cells and cells treated with the CB1 inverse agonist SR141716A. The bar graph indicated the TEER values at 24 h (B, right). Statistical differences were determined using t-test; ***p<0.001 compared to control group. Error bars, SD (n=3). (C) Morphological changes showed IEC-6 cell senescence caused by H2O2 and the restoration of cell morphology with ACEA treatment after 24 h and 48 h (n=5). Scale bar, 100 mm. (D) The decreased TEER values caused by H2O2 treatment were increased by co-treatment with the CB1 agonist ACEA. The bar graph presents the TEER values at 24 h after treatment with H2O2 and ACEA (D, right). Statistical differences were determined using t-test; ***p<0.001 compared to control group and ###p<0.001 compared to H2O2- treated group. Error bars, SD (n=3). (E, F) The mRNA expression levels of the tight junction proteins ZO-1, ZO-2, Claudin-2, Claudin-4 and Occludin were detected by RT-PCR in H2O2-treated IEC-6 cells. RT-PCR results showed a decrease in the levels of these tight junction proteins (E) and the bar graph presents the band intensity of the RT-PCR results (F). Statistical differences were determined using t-test; *p<0.05, **p<0.01, and ***p<0.001 compared to control group. Error bars, SD (n = 3). GAPDH was used for the loading control. (G) Decreased RNA expression of ZO-1 by H2O2 was restored by ACEA co-treatment. The bar graphs present the band intensity of PCR results. Statistical differences were determined using t-test; *p<0.05 compared to control group and #p<0.05 compared to H2O2-treated group. Error bars, SD (n = 3).

    Journal: Frontiers in endocrinology

    Article Title: Cannabinoid receptor type 1 in the aging gut regulates the mucosal permeability via miR-191-5p.

    doi: 10.3389/fendo.2023.1241097

    Figure Lengend Snippet: FIGURE 3 Intestinal permeability and tight junction protein expression were regulated by CB1 signaling modulation. (A) Morphological changes showed IEC-6 cell senescence by H2O2 and SR141716A treatment (n=5). Scale bar, 100 mm. (B) The TEER values were detected in H2O2-treated IEC-6 cells and cells treated with the CB1 inverse agonist SR141716A. The bar graph indicated the TEER values at 24 h (B, right). Statistical differences were determined using t-test; ***p<0.001 compared to control group. Error bars, SD (n=3). (C) Morphological changes showed IEC-6 cell senescence caused by H2O2 and the restoration of cell morphology with ACEA treatment after 24 h and 48 h (n=5). Scale bar, 100 mm. (D) The decreased TEER values caused by H2O2 treatment were increased by co-treatment with the CB1 agonist ACEA. The bar graph presents the TEER values at 24 h after treatment with H2O2 and ACEA (D, right). Statistical differences were determined using t-test; ***p<0.001 compared to control group and ###p<0.001 compared to H2O2- treated group. Error bars, SD (n=3). (E, F) The mRNA expression levels of the tight junction proteins ZO-1, ZO-2, Claudin-2, Claudin-4 and Occludin were detected by RT-PCR in H2O2-treated IEC-6 cells. RT-PCR results showed a decrease in the levels of these tight junction proteins (E) and the bar graph presents the band intensity of the RT-PCR results (F). Statistical differences were determined using t-test; *p<0.05, **p<0.01, and ***p<0.001 compared to control group. Error bars, SD (n = 3). GAPDH was used for the loading control. (G) Decreased RNA expression of ZO-1 by H2O2 was restored by ACEA co-treatment. The bar graphs present the band intensity of PCR results. Statistical differences were determined using t-test; *p<0.05 compared to control group and #p<0.05 compared to H2O2-treated group. Error bars, SD (n = 3).

    Article Snippet: Cell culture & in vitro cell senescence model Normal rat small intestinal epithelial cells (IEC-6; cat. no. CRL1592) and the human colonic epithelial adenocarcinoma cell line Caco-2 (cat. no. HTB-37TM) were purchased from American Type Culture Collection (Manassas, VA, USA).

    Techniques: Permeability, Expressing, Control, Reverse Transcription Polymerase Chain Reaction, RNA Expression

    FIGURE 7 Reduction of CB1 in aged intestinal epithelial cells caused tight junction dysfunction. Decreased CB1 is associated with the induction of miR-191-5p. miR-191-5p reduced ZO-1 expression by targeting directly and via activation of p-p65 NF-kB transcription factor.

    Journal: Frontiers in endocrinology

    Article Title: Cannabinoid receptor type 1 in the aging gut regulates the mucosal permeability via miR-191-5p.

    doi: 10.3389/fendo.2023.1241097

    Figure Lengend Snippet: FIGURE 7 Reduction of CB1 in aged intestinal epithelial cells caused tight junction dysfunction. Decreased CB1 is associated with the induction of miR-191-5p. miR-191-5p reduced ZO-1 expression by targeting directly and via activation of p-p65 NF-kB transcription factor.

    Article Snippet: Cell culture & in vitro cell senescence model Normal rat small intestinal epithelial cells (IEC-6; cat. no. CRL1592) and the human colonic epithelial adenocarcinoma cell line Caco-2 (cat. no. HTB-37TM) were purchased from American Type Culture Collection (Manassas, VA, USA).

    Techniques: Expressing, Activation Assay